Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media

• Published in: Biosensors & bioelectronics Vol. 12; no. 3; pp. 205 - 212
• Main Authors: Bedioui, Fethi, Trevin, Stéphane, Dvynck, Jacques, Lantoine, Frédérique, Brunet, Annie, Devynck, Marie-Aude
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1997; Elsevier Science
• Subjects: Biological and medical sciences; Biosensing Techniques; Biotechnology; blood platelets; Blood Platelets - metabolism; Cells, Cultured; Culture Media - chemistry; electrochemical detection; Electrochemistry - instrumentation; Electrochemistry - methods; endothelial cells; Endothelium, Vascular - metabolism; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Methods. Procedures. Technologies; Nickel; nitric oxide; Nitric Oxide - analysis; Nitric Oxide - biosynthesis; Others; porphyrinic sensor; ultramicroelectrodes; Various methods and equipments; electrochemical detection; nitric oxide; blood platelets; endothelial cells; ultramicroelectrodes; porphyrinic sensor; Endothelial cell; Chemical modification; Platelet; Metallophthalocyanine; Nitric oxide; Carbon electrode; Nickel; Ultramicroelectrode; Umbilical vein; Medical application; Blood; Electrochemical detector
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(97)85338-9

We described here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein.