Isolation, characterization, and chromosome mapping of a human A-C1 Ha-Ras suppressor gene (HRASLS)

• Published in: Cytogenetic and genome research Vol. 93; no. 1-2; pp. 36 - 39
• Main Authors: Ito, H., Akiyama, H., Shigeno, C., Nakamura, T.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2001; S. Karger AG
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; chromosome 3; Chromosome Banding; Chromosomes, Human, Pair 3 - genetics; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Mapping, Cloning and Sequencing; Genes, ras - genetics; Genetics of eukaryotes. Biological and molecular evolution; HRASLS gene; Human; Humans; In Situ Hybridization, Fluorescence; Male; Mice; Molecular Sequence Data; Muscle, Skeletal - metabolism; Organ Specificity; Physical Chromosome Mapping; Proteins - chemistry; Proteins - genetics; RNA, Messenger - analysis; RNA, Messenger - genetics; Sequence Alignment; Sequence Homology; Suppression, Genetic - genetics; Testis - metabolism; Tumor Cells, Cultured; Genetic mapping; Human; Nucleotide sequence; Diseases of the osteoarticular system; Homology; Chromosome A3; Malignant tumor; Gene expression; Signal transduction; Regulation(control); Osteosarcoma; C-Onc gene; Bone; Physical map
• ISSN: 1424-8581; 0301-0171; 1424-859X
• DOI: 10.1159/000056944

Recently, we cloned a cDNA encoding a novel mouse protein, named A-C1, by differential display between two mouse cell lines, embryonic fibroblast C3H10T1/2 and chondrogenic ATDC5. Mouse A-C1 has homology with a ras-responsive gene, rat Ha-rev107 (Hrasls), and modulates a Ha-ras-mediated signaling pathway. Here, we report a cDNA encoding a human homolog of mouse A-C1. The deduced amino acid sequence of human A-C1 consists of 168 amino acids, and shows 83% identity with that of mouse A-C1. Human A-C1 mRNA was expressed in skeletal muscle, testis, heart, brain, and thyroid in vivo. Moreover, expression of human A-C1 mRNA was detected at a high level in human osteosarcoma-derived U2OS cells in vitro. By FISH analysis the human A-C1 gene (HRASLS) was mapped to human chromosome 3q28→ q29.