All or none block of single Na+ channels by tetrodotoxin

The effects of tetrodotoxin on single Na+-channel currents recorded from excised patches of neuroblastoma cells were examined. Tetrodotoxin was found to cause a dose-dependent reduction in the frequency at which Na+ channels conduct during a series of depolarizations. Surviving conducting states had...

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Bibliographic Details
Published inNeuroscience letters Vol. 54; no. 1; p. 77
Main Authors Quandt, F N, Yeh, J Z, Narahashi, T
Format Journal Article
LanguageEnglish
Published Ireland 28.02.1985
Subjects
Cell Line
Ion Channels - drug effects
Membrane Potentials
Models, Neurological
Neuroblastoma
Saxitoxin - pharmacology
Sodium - metabolism
Tetrodotoxin - pharmacology
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Summary:The effects of tetrodotoxin on single Na+-channel currents recorded from excised patches of neuroblastoma cells were examined. Tetrodotoxin was found to cause a dose-dependent reduction in the frequency at which Na+ channels conduct during a series of depolarizations. Surviving conducting states had normal open times and current amplitudes. These effects could be explained by a model which includes initial binding of tetrodotoxin to a closed state of the channel with stable, complete block during the time the channel would normally be gated open.
ISSN:0304-3940
DOI:10.1016/s0304-3940(85)80121-x