Bladder carcinoma producing granulocyte colony-stimulating factor: a case report

• Published in: The Journal of urology Vol. 149; no. 4; p. 843
• Main Authors: Satoh, H, Abe, Y, Katoh, Y, Komine, Y, Nakamura, M, Tamaoki, N
• Format: Journal Article
• Language: English
• Published: United States 01.04.1993
• Subjects: Aged; Blotting, Northern; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - secondary; Gene Expression; Granulocyte Colony-Stimulating Factor - genetics; Granulocyte Colony-Stimulating Factor - metabolism; Humans; Immunoenzyme Techniques; Leukocytosis - etiology; Male; Paraneoplastic Syndromes - etiology; RNA, Neoplasm - genetics; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology
• ISSN: 0022-5347
• DOI: 10.1016/S0022-5347(17)36229-8

A 67-year-old man with squamous cell bladder carcinoma showed remarkable leukocytosis (maximum 50,300 per microliter.) for 4 months while the neoplasm recurred and metastasis progressed. The patient died of systemic metastasis within 9 months after cystectomy. Enzyme immunoassay of the serum demonstrated remarkably high levels of granulocyte colony-stimulating factor (4,928 pg./ml.). Immunohistochemical examination with anti-granulocyte colony-stimulating factor monoclonal antibody demonstrated granulocyte colony-stimulating factor production in the metastatic cancer cells in the liver. The expression of the granulocyte colony-stimulating factor gene in the tumor specimens was examined by standard Northern blot analysis. Specific granulocyte colony-stimulating factor transcript was identified in the total ribonucleic acid fraction extracted from a tumor specimen of the peritoneal metastatic lesion. These results indicated that the autonomous production of granulocyte colony-stimulating factor in this bladder carcinoma induced paraneoplastic leukocytosis.