Detection and characterization of pediatric serum antibody to the OMP P5-homologous adhesin of nontypeable Haemophilus influenzae during acute otitis media

• Published in: Vaccine Vol. 20; no. 29; pp. 3590 - 3597
• Main Authors: Novotny, Laura A, Pichichero, Michael E, Denoël, Philippe A, Neyt, Cecil, Vanderschrick, Sylvie, Dequesne, Guy, Bakaletz, Lauren O
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 04.10.2002; Elsevier
• Subjects: Acute Disease; Adhesins, Bacterial - immunology; Antibodies, Bacterial - blood; Bacterial Outer Membrane Proteins - analysis; Bacterial Outer Membrane Proteins - immunology; Bacteriology; Biological and medical sciences; Biosensor; Child; Child, Preschool; Epidemiology. Vaccinations; Epitope-targeted vaccine; Fimbriae, Bacterial; Fundamental and applied biological sciences. Psychology; General aspects; Haemophilus influenzae; Haemophilus influenzae - immunology; Humans; Infant; Infectious diseases; Medical sciences; Microbiology; Otitis Media - immunology; Otitis Media - microbiology; Rodentia; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Australia, Queensland, Chinchilla; Epitope-targeted vaccine; Biosensor; Human; Antibody; Middle ear disease; Vaccine; Haemophilus influenzae; Pasteurellaceae; Characterization; Bacteria; ENT disease; Otitis media; Serum; Adhesin; Detection; Child
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(02)00306-7

We reported earlier that antibody in sera collected from seven children with acute otitis media (AOM) due to nontypeable Haemophilus influenzae (NTHI) recognized immunodominant regions of P5-fimbrin just as we had observed in a chinchilla model of experimental NTHI-induced AOM. To expand upon those preliminary findings, we further characterized pediatric serum antibodies directed against this adhesin during AOM. Collectively, the data show that children respond immunologically to P5-fimbrin and they do so in a manner that allows for the distinction of sequence diversity within short linear peptides representing a focused region of this surface-exposed protein. The immune recognition we observed encourages us to further develop a P5-fimbrin based vaccine component.