Micellar electrokinetic chromatography for the determination of urinary desmosine and isodesmosine in patients affected by chronic obstructive pulmonary disease

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 714; no. 1; pp. 87 - 98
• Main Authors: Viglio, Simona, Zanaboni, Giuseppe, Luisetti, Maurizio, Trisolini, Rocco, Grimm, Rudi, Cetta, Giuseppe, Iadarola, Paolo
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 28.08.1998; Elsevier Science
• Subjects: Biological and medical sciences; Biomarkers - urine; Chromatography, Micellar Electrokinetic Capillary; Chronic obstructive pulmonary disease, asthma; Desmosine; Desmosine - urine; Humans; Isodemosine; Isodesmosine - urine; Lung Diseases, Obstructive - urine; Medical sciences; Pneumology; Spectrophotometry, Ultraviolet; Desmosine; Isodemosine; Human; Urine; Biological fluid; Lung disease; Respiratory disease; Micellar electrokinetic capillary chromatography; Biological marker; Desmosin; Aminoacid; Laboratory investigations; Clinical biology; Simultaneous measurement; Bronchus disease; Obstructive pulmonary disease; Isodemosin; Quantitative analysis
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(98)00046-2

The presence in urine of desmosine (DES) and isodesmosine (IDES), two crosslinked amino acids unique to the elastic fiber network, can be used as a specific indicator of degradation of mature elastin. Compared to methodologies so far available, the capillary electrophoretic technique reported here seems to be suitable and convenient for determining desmosines in urine of patients affected by chronic obstructive pulmonary disease (COPD). By using 35 m M sodium tetraborate pH 9.3 containing 65 m M SDS as the background electrolyte, the peaks of DES and IDES could be detected in hydrolyzed urine samples from controls and patients. Owing to the simultaneous determination of endogenous urinary creatinine used as appropriate internal standard, the amount of these amino acids could be accurately quantified. The results obtained were of the same order of magnitude as the data already reported in the literature for COPD patients. Thus micellar electrokinetic chromatography (MEKC) may be considered as a reliable technique for studying the turnover of the elastic fiber in clinical conditions.