Identification of the 64 kilodalton chloroplast stromal phosphoprotein as phosphoglucomutase

• Published in: Plant physiology (Bethesda) Vol. 93; no. 1; pp. 105 - 109
• Main Authors: Salvucci, M.E. (USDA, ARS, Lexington, KY), Drake, R.R, Broadbent, K.P, Haley, B.E, Hanson, K.R, McHale, N.A
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.1990
• Subjects: 550201 - Biochemistry- Tracer Techniques; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agriculture; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; Biological and medical sciences; Cell biochemistry; CELL CONSTITUENTS; Cell physiology; CHEMICAL REACTIONS; CHLOROPLASTS; DAYS LIVING RADIOISOTOPES; Electrophoresis; ENZYME ACTIVITY; ENZYMES; FOSFORILACION; Fundamental and applied biological sciences. Psychology; Gels; ISOTOPE APPLICATIONS; ISOTOPES; LEGUMINOSAE; LIGHT NUCLEI; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METABOLITES; MUTANT; MUTANTES; NICOTIANA; NUCLEI; ODD-ODD NUCLEI; Peas; PEPTIDE; PEPTIDOS; Phosphoproteins; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; PHOSPHORYLATION; PHOSPHOTRANSFERASES; PISUM; PISUM SATIVUM; Plant physiology and development; PLANTS; PLASTE; PLASTIDIOS; Plastids; RADIOISOTOPES; Spinach; TRACER TECHNIQUES; TRANSFERASAS; TRANSFERASE; TRANSFERASES; Phosphorylation; Radiolabelling; Enzyme; Phosphate sugar; Phosphoglucomutase; Leguminosae; Phosphoproteins; Dicotyledones; Pisum sativum; Angiospermae; Spermatophyta; Solanaceae; ATP; Chloroplast
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.93.1.105

Phosphorylation of the 64 kilodalton stromal phosphoprotein by incubation of pea (Pisum sativum) chloroplast extracts with [gamma-32P]ATP decreased in the presence of Glc-6-P and Glc-1,6-P2, but was stimulated by glucose. Two-dimensional gel electrophoresis following incubation of intact chloroplasts and stromal extracts with [gamma-32P]ATP, or incubation of stromal extracts and partially purified phosphoglucomutase (EC 2.7.5.1) with [32P]Glc-1-P showed that the identical 64 kilodalton polypeptide was labeled. A 62 kilodalton polypeptide was phosphorylated by incubation of tobacco (Nicotiana sylvestris) stromal extracts with either [gamma-32P]ATP or [32P]Glc-l-P. In contrast, an analogous polypeptide was not phosphorylated in extracts from a tobacco mutant deficient in plastid phosphoglucomutase activity. The results indicate that the 64 (or 62) kilodalton chloroplast stromal phosphoprotein is phosphoglucomutase