Automated determination of levodopa and carbidopa in plasma by high-performance liquid chromatography-electrochemical detection using an on-line flow injection analysis sample pretreatment unit

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 698; no. 1; pp. 201 - 207
• Main Authors: Tolokán, A., Klebovich, I., Balogh-Nemes, K., Horvai, G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.09.1997; Elsevier Science
• Subjects: Analysis; Animals; Antiparkinson Agents - blood; Automation; Biological and medical sciences; Carbidopa; Carbidopa - blood; Chromatography, High Pressure Liquid - methods; Dogs; Electrochemistry; Flow Injection Analysis; General pharmacology; Levodopa; Levodopa - blood; Male; Medical sciences; Methyldopa - blood; Pharmacology. Drug treatments; Reference Standards; Carbidopa; Levodopa; Fissipedia; Biological fluid; Carnivora; Automation; Decarboxylase; Enzyme; Oral administration; HPLC chromatography; Enzyme inhibitor; Lyases; Antiparkinson agent; Blood; Electrochemical detector; Blood plasma; Carbon-carbon lyases; Vertebrata; Mammalia; Carboxy-lyases; Simultaneous measurement; Dog; Quantitative analysis; Flow injection

An automated analytical procedure is described for the parallel determination of l-3,4-dihydroxyphenylalanine (levodopa, l-dopa, LD) and the analogous hydrazine compound carbidopa (CD) in dog plasma by ion-pair high-performance liquid chromatography with electrochemical detection (HPLC-ED). After de...