Automated determination of levodopa and carbidopa in plasma by high-performance liquid chromatography-electrochemical detection using an on-line flow injection analysis sample pretreatment unit

An automated analytical procedure is described for the parallel determination of l-3,4-dihydroxyphenylalanine (levodopa, l-dopa, LD) and the analogous hydrazine compound carbidopa (CD) in dog plasma by ion-pair high-performance liquid chromatography with electrochemical detection (HPLC-ED). After de...

Full description

Saved in:
Bibliographic Details
Published inJournal of chromatography. B, Biomedical sciences and applications Vol. 698; no. 1; pp. 201 - 207
Main Authors Tolokán, A., Klebovich, I., Balogh-Nemes, K., Horvai, G.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 26.09.1997
Elsevier Science
Subjects
Analysis
Animals
Antiparkinson Agents - blood
Automation
Biological and medical sciences
Carbidopa
Carbidopa - blood
Chromatography, High Pressure Liquid - methods
Dogs
Electrochemistry
Flow Injection Analysis
General pharmacology
Levodopa
Levodopa - blood
Male
Medical sciences
Methyldopa - blood
Pharmacology. Drug treatments
Reference Standards
Carbidopa
Levodopa
Fissipedia
Biological fluid
Carnivora
Automation
Decarboxylase
Enzyme
Oral administration
HPLC chromatography
Enzyme inhibitor
Lyases
Antiparkinson agent
Blood
Electrochemical detector
Blood plasma
Carbon-carbon lyases
Vertebrata
Mammalia
Carboxy-lyases
Simultaneous measurement
Dog
Quantitative analysis
Flow injection
Online AccessGet full text

Cover

More Information
Summary:An automated analytical procedure is described for the parallel determination of l-3,4-dihydroxyphenylalanine (levodopa, l-dopa, LD) and the analogous hydrazine compound carbidopa (CD) in dog plasma by ion-pair high-performance liquid chromatography with electrochemical detection (HPLC-ED). After deproteinization of the plasma samples with perchloric acid the catecholamines were extracted from the supernatant by adsorption on a small column filled with alumina. The extraction and redissolution were automatically performed in a flow injection analysis unit (FIA) coupled to the HPLC system. The performance of the whole system was tested on dog plasma samples including specimens taken after oral administration of the anti-Parkinsonism drug Duellin ®, which is a combination tablet of levodopa and carbidopa.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00288-0