The Quantitation of 2-Oxo-3-hydroxy Lysergic Acid Diethylamide (O-H-LSD)in Human Urine Specimens, a Metabolite of LSD: Comparative Analysis Using Liquid Chromatography-Selected Ion Monitoring Mass Spectrometry and Liquid Chromatography-Ion Trap Mass Spectrometry

• Published in: Journal of analytical toxicology Vol. 24; no. 3; pp. 170 - 179
• Main Authors: Poch, Gregory K., Klette, Kevin L., Anderson, Cynthia
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.04.2000; Preston
• Subjects: 2-oxo-3-hydroxy lysergic acid; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug addictions; Evaluation Studies as Topic; Gas Chromatography-Mass Spectrometry - methods; Humans; lysergic acid; Lysergic Acid Diethylamide - analogs & derivatives; Lysergic Acid Diethylamide - urine; lysergide; Medical sciences; Reproducibility of Results; Toxicology; Human; Urine; Solid phase extraction; Biological fluid; Chemical analysis; Atmospheric pressure; Liquid liquid extraction; Metabolite; Coupled method; Psychotropic; HPLC chromatography; Forensic aspect; Lysergide; Solvent extraction; Chemical ionization; Ion trap; Sample preparation; Mass spectrometry MS/MS; Hallucinogen; Drug of abuse; Mass spectrometry; Comparative study; Quantitative analysis
• ISSN: 0146-4760; 1945-2403
• DOI: 10.1093/jat/24.3.170

This paper compares the potential forensic application of two sensitive and rapid procedures (liquid chromatography-mass spectrometry and liquid chromatography-ion trap mass spectrometry) for the detection and quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) a major LSD metabolite. O-H-LSD calibration curves for both procedures were linear over the concentration range 0-8000 pg/mL with correlation coefficients (r2) greater than 0.99. The observed limit of detection (LOD) and limit of quantitation (LOQ) for O-H-LSD in both procedures was 400 pg/mL. Sixty-eight human urine specimens that had previously been found to contain LSD by gas chromatography-mass spectrometry were reanalyzed by both procedures for LSD and O-H-LSD. These specimens contained a mean concentration of O-H-LSD approximately 16 times higher than the LSD concentration. Because both LC methods produce similar results, either procedure can be readily adapted to O-H-LSD analysis for use in high-volume drug-testing laboratories. In addition, the possibility of significantly increasing the LSD detection time window by targeting this major LSD metabolite for analysis may influence other drug-free workplace programs to test for LSD.