Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus

•A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV.•The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR.•Blood samples could be directly applied as PCR template in iiPCR without DNA extraction....

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Published inVirologica Sinica Vol. 37; no. 3; pp. 462 - 464
Main Authors Zou, Tianli, Deng, Junhua, Li, Xiangdong, Zhang, Shiyin, Chen, Lingyan, Hao, Liying, Zhuang, Jinshan, Wang, Heng, Zhang, Guihong, Ge, Shengxiang, Tian, Kegong
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2022
Wuhan Institute of Virology, Chinese Academy of Sciences
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Letter
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Summary:•A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV.•The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR.•Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.
Bibliography:SourceType-Other Sources-1
content type line 63
ObjectType-Correspondence-1
Tianli Zou, Junhua Deng, and Xiangdong Li contributed equally to this work.
ISSN:1995-820X
1674-0769
1995-820X
DOI:10.1016/j.virs.2022.03.002