Platelet-activating factor in normal rat uterus

• Published in: Biochemical and biophysical research communications Vol. 138; no. 3; pp. 1231 - 1236
• Main Authors: Yasuda, Katsuhiko, Satouchi, Kiyoshi, Saito, Kunihiko
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 14.08.1986; Elsevier
• Subjects: Animals; Applied sciences; Exact sciences and technology; Fatty Alcohols - analysis; Female; Gas Chromatography-Mass Spectrometry; Other techniques and industries; Plasmalogens - metabolism; Platelet Activating Factor - isolation & purification; Platelet Activating Factor - metabolism; Platelet Activating Factor - pharmacology; Platelet Aggregation - drug effects; Rats; Uterus - physiology; 16:0 AGEPC; GC-MS; 16:0 lysoGEPC; PAF; tBDMS; SIM
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(86)80414-4

Platelet-activating factor (PAF) was found in normal rat uterus and identified as 1-O-hexadecyl/octadecenyl-2-acetyl- sn-glycero-phosphocholine. PAF was purified by several successive chromatographic procedures. It showed platelet aggregating activity, which was inhibited by CV 3988, and had no effect on platelets desensitized with 1-0-hexadecyl-2-acetyl- sn-glycero-3-phosphocholine. The tert-butyldimethyl-silylderivative of 1-O-alkyl-2-acetyl- sn-glycerol, which was obtained by hydrolysis of uterine PAF with phospholipase C, was analyzed by gas chromatography-mass spectrometry. One rat uterus contained approximately 21.3 ng of 1-O-hexadecyl-2-acetyl- sn-glycero-3-phosphocholine. This is the first report of the occurrence of a significant amount of PAF in a normal animal tissue.