Characterization of an Iron-responsive Promoter in the Protozoan Pathogen Trichomonas vaginalis
Iron has been shown to regulate transcription in the protozoan pathogen Trichomonas vaginalis . In this study, a DNA transfection system was developed to monitor ap65-1 promoter activity in response to changing iron supply. In conjunction with electrophoretic mobility shift assay, iron-induced trans...
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Published in | The Journal of biological chemistry Vol. 277; no. 7; pp. 5153 - 5162 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
15.02.2002
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Subjects | |
Online Access | Get full text |
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Summary: | Iron has been shown to regulate transcription in the protozoan pathogen Trichomonas vaginalis . In this study, a DNA transfection system was developed to monitor ap65-1 promoter activity in response to changing iron supply. In conjunction with electrophoretic mobility shift assay, iron-induced
transcription of the ap65-1 gene was shown to be regulated by multiple closely spaced DNA elements spanning an iron-responsive region (â110/â54), including
an iron-responsive DNA element ( â98 AGATAACGA â90 ), which overlaps with a 3â²-MYB-like protein binding sequence ( â95 TAACGATAT â87 ), and three nearby T-rich sequences ( â110 ATTTTT â105 , â78 ATTATT â73 , and â59 ATTTTT â54 ). 5â²- and 3â²-flanking sequences of the iron-responsive region were shown to regulate basal transcription. A distal DNA regulatory
region was shown to enhance both basal and iron-induced transcription. These findings delineate the DNA regulatory elements
and nuclear proteins involving in iron-induced transcription of the ap65-1 gene, which provide useful tools for the future study of transcriptional regulation in T. vaginalis . |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M110234200 |