A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

• Published in: Biosensors (Basel) Vol. 12; no. 7; p. 456
• Main Authors: Lu, Yi-Kuan, Xu, Di, Liu, Wen-Yue, Xie, Jing, Lu, Ying
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26.06.2022; MDPI
• Subjects: Antigens; Aquaculture; Au nanoparticles; Chromatography; Fish; Fisheries; Fishing; High-performance liquid chromatography; Liquid chromatography; Malachite green; Methods; Nanoparticles; Quantum dots; Residues; Shellfish; simultaneous detection; tricaine mesylate; tricolour test strip; United States > US; China; Germany
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios12070456

In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (~20 min, HPLC~3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products.