Development of a Prototype Lateral Flow Immunoassay of Cortisol in Saliva for Daily Monitoring of Stress

Emotional stress negatively affects the quality of a person’s daily life. From a physiological point of view, stress is expressed in the excitation of the hypothalamic–pituitary–adrenal cortex axis, which leads to the release of the hormone cortisol into the blood. We developed a lateral flow immuno...

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Bibliographic Details
Published inBiosensors (Basel) Vol. 11; no. 5; p. 146
Main Author Panfilova, Elizaveta
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 07.05.2021
MDPI
Subjects
Adrenal cortex
Antibodies
Anxiety disorders
Calibration
Cortisol
emotional stress
Enzyme-linked immunosorbent assay
gold nanoparticles conjugation
Hormones
Hypothalamus
Immunoassay
immunochromatographic test
Medical research
Pituitary
point-of-care testing
rapid detection
Reagents
Saliva
salivatory cortisol
Stress
United States > US
Russia
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Summary:Emotional stress negatively affects the quality of a person’s daily life. From a physiological point of view, stress is expressed in the excitation of the hypothalamic–pituitary–adrenal cortex axis, which leads to the release of the hormone cortisol into the blood. We developed a lateral flow immunoassay to detect cortisol in human salivary fluid and tested it on 10 healthy volunteers daily for about one month (n = 293 saliva samples). Cortisol was detected in concentrations ranging from 1 to 70 ng/mL. Salivary cortisol levels were confirmed by ELISA. The straightness range of LFIA calibration was from 1 to 100 ng/mL. The diagnostic sensitivity of the method was 73%. It was found that in 3 out of 10 subjects, fluctuations in the level of cortisol in saliva partially corresponded to the subjectively assessed level of stress.
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ISSN:2079-6374
2079-6374
DOI:10.3390/bios11050146