Effect of Chronologic Age on Induction of Cystathionine Synthase, Uroporphyrinogen I Synthase, and Glucose-6-phosphate Dehydrogenase Activities in Lymphocytes

The activities of cystathionine synthase [L-serine hydro-lyase (adding homocysteine), EC 4.2.1.22], uroporphyrinogen I synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8], and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+1-oxidoreductase, EC 1.1.1.49) have been meas...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 78; no. 3; pp. 1916 - 1919
Main Authors Gartler, Stanley M., Hornung, Sigrid K., Motulsky, Arno G.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.03.1981
National Acad Sciences
Subjects
Adult
Aged
Aging
Ammonia-Lyases - biosynthesis
Cultured cells
Cystathionine beta-Synthase - biosynthesis
Cystathionine beta-Synthase - blood
Dehydrogenases
Enzyme activity
Enzyme Induction
Enzymes
Glucosephosphate Dehydrogenase - biosynthesis
Glucosephosphate Dehydrogenase - blood
Homocystinuria
Humans
Hydro-Lyases - biosynthesis
Hydroxymethylbilane Synthase - biosynthesis
Hydroxymethylbilane Synthase - blood
Leukocytes
Lymphocytes
Lymphocytes - enzymology
Metabolic diseases
Middle Aged
Mitogens
Uroporphyrinogens
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Summary:The activities of cystathionine synthase [L-serine hydro-lyase (adding homocysteine), EC 4.2.1.22], uroporphyrinogen I synthase [porphobilinogen ammonia-lyase (polymerizing), EC 4.3.1.8], and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+1-oxidoreductase, EC 1.1.1.49) have been measured in phytohemagglutinin-stimulated lymphocytes of young and old human subjects. A significant decrease in activity with age was observed for cystathionine synthase and uroporphyrinogen I synthase but not for glucose-6-phosphate dehydrogenase. These changes could not be related to declining phytohemagglutinin response with aging. Age-related decreases in activity of some enzymes may be relevant for an understanding of the biology of aging. False assignment of heterozygosity, and even homozygosity, for certain genetic disorders, such as homocystinuria, may result when low enzyme levels are detected in the lymphocytes of older people.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.3.1916