The formulation, characterization and in vivo evaluation of a magnetic carrier for brain delivery of NIR dye

• Published in: Nanotechnology Vol. 21; no. 39; p. 395102
• Main Authors: Raut, S L, Kirthivasan, B, Bommana, M M, Squillante, E, Sadoqi, M
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.10.2010
• Subjects: Analysis of Variance; Animals; Brain; Brain Chemistry; Computational efficiency; Computing time; Contrast Media - chemistry; Contrast Media - pharmacokinetics; Drug Delivery Systems; Dyes; Encapsulation; Formulations; Histocytochemistry; Indocyanine Green - chemistry; Indocyanine Green - pharmacokinetics; Lactic Acid; Magnetic fields; Magnetite Nanoparticles - chemistry; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanocomposites - chemistry; Nanoparticles; Oleic Acid; Particle Size; Polyglycolic Acid; Rats; Rats, Sprague-Dawley; Spectroscopy, Fourier Transform Infrared; Spectroscopy, Near-Infrared - methods
• ISSN: 0957-4484; 1361-6528
• DOI: 10.1088/0957-4484/21/39/395102

This work reports the targeting of the near infrared (NIR) dye indocyanine green (ICG) to the brain using composite nanoparticles. Thermal decomposition of iron pentacarbonyl was used to synthesize monodisperse oleic acid coated magnetic nanoparticles (OAMNP). Synthesized OAMNP and ICG were encapsulated in a poly (lactide-co-glycolide) matrix using an emulsion evaporation method. Different batches containing OAMNP:PLGA ratios (1:4, 1:2 and 3:4) were prepared with ICG (group B-1, 2, 3) and without ICG (group A-1, 2, 3) loading. All the formulations were characterized in terms of morphology, particle size, zeta potential, magnetic content, ICG encapsulation efficiency and the spectral properties of ICG. The optimized formulation showed an encapsulation efficiency of 56 +/- 4.6% for ICG and 57 +/- 1.37% for OAMNP. The biodistribution and brain targeting study involved three groups of six animals, each with 0.4 mg kg(-1) equivalent of ICG, given as neat ICG solution, composite nanoparticles without the aid of a magnetic field, and composite nanoparticles under the influence of a magnetic field (8000 G) to groups 1, 2 and 3 respectively. The tissue analysis and microscopy images revealed a significantly higher brain concentration of ICG (p < 0.05) for group 3 than the two control groups. These results are encouraging for the brain delivery of hydrophilic dyes/drugs using this method for biomedical applications.