DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes

Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery and degradation by endo-lysosomes is a tightly regulated process and important for maintaining cellu...

Full description

Saved in:
Bibliographic Details
Published inBio-protocol Vol. 7; no. 19
Main Authors Marwaha, Rituraj, Sharma, Mahak
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.10.2017
Bio-protocol LLC
Subjects
Biology
Methods
HeLa
Cargo
Cell culture
Endocytosis
DQ-BSA
Lysosome
Online AccessGet full text

Cover

More Information
Summary:Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery and degradation by endo-lysosomes is a tightly regulated process and important for maintaining cellular homeostasis. Cargos like EGF (Epidermal Growth Factor), Dil-LDL (3,3'-Dioctadecylindocarbocyanine-Low Density Lipoprotein), Dextran, DQ-BSA (Dye Quenched-Bovine Serum Albumin) are routinely used by researchers to analyze the role of various proteins in endocytic pathway. Trafficking of DQ-BSA in cells depleted of or over-expressing the gene of interest is a useful assay for identifying the role of various proteins in endocytic trafficking pathway. The protocol describes the DQ-Red BSA trafficking assay that can be used to study endocytic trafficking in various cell types.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2331-8325
2331-8325
DOI:10.21769/bioprotoc.2571