Improved analytical procedure for the measurement of captopril in human plasma by gas chromatography–mass spectrometry and its application to pharmacokinetic studies
An enhanced, sensitive GC–MS assay is presented for the highly specific angiotensin-converting enzyme (ACE) inhibitor, captopril. This method improves previously published assays by using solid NEM as stabilizer in the collection tubes, a rapid extraction technique with dichloromethane and back-extr...
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Published in | Journal of chromatography. B, Biomedical sciences and applications Vol. 705; no. 1; pp. 47 - 54 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
23.01.1998
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | An enhanced, sensitive GC–MS assay is presented for the highly specific angiotensin-converting enzyme (ACE) inhibitor, captopril. This method improves previously published assays by using solid NEM as stabilizer in the collection tubes, a rapid extraction technique with dichloromethane and back-extraction into base, a commercially available internal standard (thiosalicylic acid) and a capillary GC column. Captopril and the internal standard are measured as their bis-pentafluorobenzyl derivatives. The assay was linear from 10 to 5000 ng/ml with a mean recovery following solvent extraction at 50, 200 and 1000 ng/ml of 77%. At mean values of 45.9, 187 and 980 ng/ml inter-assay precision and accuracy were 4.0, 2.9 and 3.5% and 8.2, 6.5 and 3.1%, respectively. Analysis of captopril concentrations in plasma samples from 20 volunteers following oral administration of 100 mg of captopril provided the following pharmacokinetic data (mean±S.D.):
C
max, 1470±467 ng/ml; AUC
0–∞, 1736±481 ng/ml.h;
T
max, 0.73 h;
k
e, 0.468±0.122 h
−1; elimination half life, 1.58±0.41 h. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-4347 1387-2273 |
DOI: | 10.1016/S0378-4347(97)00481-7 |