Transcriptome profiling in Arabidopsis inflorescence stems grown under hypergravity in terms of cell walls and plant hormones

• Published in: Advances in space research Vol. 44; no. 2; pp. 245 - 253
• Main Authors: Tamaoki, D., Karahara, I., Nishiuchi, T., De Oliveira, S., Schreiber, L., Wakasugi, T., Yamada, K., Yamaguchi, K., Kamisaka, S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 15.07.2009; Elsevier
• Subjects: Arabidopsis; Arabidopsis thaliana; Astronomy; Auxin; Cell wall; Earth, ocean, space; Exact sciences and technology; External geophysics; Hypergravity; Lignin; Microarray; EXT; Lignin; IAOx; Auxin; GRP; LRP; Microarray; XTH; Cell wall; Trp; C4H; Cy; RT-PCR; PBP; ACC; GUS; Arabidopsis; GO; IAA; AGP; PID; FAH; Hypergravity; ACO; HEL; ATPA2; JA; TCH; C3H; Space research; Gravity
• ISSN: 0273-1177; 1879-1948
• DOI: 10.1016/j.asr.2009.03.016

Land plants rely on lignified secondary cell walls in supporting their body weight on the Earth. Although gravity influences the formation of the secondary cell walls, the regulatory mechanism of their formation by gravity is not yet understood. We carried out a comprehensive analysis of gene expression in inflorescence stems of Arabidopsis thaliana L. using microarray (22 K) to identify genes whose expression is modulated under hypergravity condition (300 g). Total RNA was isolated from the basal region of inflorescence stems of plants grown for 24 h at 300 g or 1 g. Microarray analysis showed that hypergravity up-regulated the expression of 403 genes to more than 2-fold. Hypergravity up-regulated the genes responsible for the biosynthesis or modification of cell wall components such as lignin, xyloglucan, pectin and structural proteins. In addition, hypergravity altered the expression of genes related to the biosynthesis of plant hormones such as auxin and ethylene and that of genes encoding hormone-responsive proteins. Our transcriptome profiling indicates that hypergravity influences the formation of secondary cell walls by modulating the pattern of gene expression, and that auxin and/or ethylene play an important role in signaling hypergravity stimulus.