miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts

Proliferation, differentiation, and apoptosis are three essential stages in cell development, and miRNAs can achieve extensive regulation of cellular developmental processes by repressing the expression of target genes. According to our previous RNA-seq results, miRNA-10a-5p was differentially expre...

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Published inInternational journal of molecular sciences Vol. 23; no. 17; p. 9545
Main Authors Zhang, Genxi, Zhang, Xinchao, Zhou, Kaizhi, Ling, Xuanze, Zhang, Jin, Wu, Pengfei, Zhang, Tao, Xie, Kaizhou, Dai, Guojun
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2022
MDPI
Subjects
Adipocytes
Apoptosis
Autophagy
Bcl-6 protein
BCL6
Cell cycle
Cell growth
Chromosome 5
Differentiation
Experiments
Flow cytometry
Gene expression
Lymphoma
MicroRNAs
miRNA
miRNA-10a-5p
Muscles
Musculoskeletal system
Myoblasts
Ovarian cancer
Poultry
proliferation
Prostate cancer
Protein expression
Proteins
Skeletal muscle
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Summary:Proliferation, differentiation, and apoptosis are three essential stages in cell development, and miRNAs can achieve extensive regulation of cellular developmental processes by repressing the expression of target genes. According to our previous RNA-seq results, miRNA-10a-5p was differentially expressed at different periods in chicken myoblasts, revealing a possible association with muscle development. In this study, we concluded that miRNA-10a-5p inhibited chicken myoblasts’ proliferation and differentiation and promoted chicken myoblasts’ apoptosis by directly targeting BCL6, a critical transcription factor involved in muscle development and regeneration. Overexpression of BCL6 significantly facilitated myoblasts’ proliferation and differentiation and suppressed myoblasts’ apoptosis. On the contrary, knockdown of BCL6 significantly repressed myoblasts’ proliferation and differentiation and induced myoblasts’ apoptosis. The results above suggest that miRNA-10a-5p plays a potential role in skeletal muscle growth, development and autophagy by targeting the BCL6 gene. We first revealed the functions of miRNA-10a-5p and BCL6 in the proliferation, differentiation, and apoptosis of chicken myoblasts.
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These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23179545