AD-MSCs and BM-MSCs Ameliorating Effects on The Metabolic and Hepato-renal Abnormalities in Type 1 Diabetic Rats

• Published in: Saudi journal of biological sciences Vol. 29; no. 2; pp. 1053 - 1060
• Main Authors: El-Sawah, Shady G., Rashwan, Hanan M., Althobaiti, Fayez, Aldhahrani, Adil, Fayad, Eman, Shabana, El-Shaimaa, El-Hallous, Ehab I., Amen, Rehab M.
• Format: Journal Article
• Language: English
• Published: Saudi Arabia Elsevier B.V 01.02.2022; Elsevier
• Subjects: Diabetes; Diabetic nephropathy; Hyperlipidemia; MSCs; Original; T1DM; D; ALP; LDL-C; ALT; DM; DN; FBG; HO-1; LPO; HDL-C; DMEM; MSCs; IPCs; FBS; STZ; Diabetic nephropathy; BUN; SPSS; HbA1c; PBS; CD; AST; Hyperlipidemia; BM-MSCs; γ-GT; AGEs; TC; TG; ROS; TL; ISCT; SEM; Diabetes; AD-MSCs; D, Diabetic; ISCT, International Society for Cellular Therapy; SPSS, Statistical Package for Social Scientists; AD-MSCs, Adipose-derived mesenchymal stem cells; HDL-C, High-density lipoprotein cholesterol; LDL-C, Low-density lipoprotein cholesterol; DM, Diabetes mellitus; IPCs, Insulin producing cells; TG, Triglycerides; DMEM, Dulbecco's modified Eagle's medium; HO-1, Heme-oxygenase 1; BM-MSCs, Bone marrow-derived mesenchymal stem cells; FBG, Fasting blood glucose; γ-GT, gamma glutamyl transferase; TC, Total cholesterol; TL, Total lipids; AGEs, Advanced glycation end products; STZ, Streptozotocin; HbA1c, Glycosylated hemoglobin; DN, Diabetic nephropathy; LPO, Lipid peroxidation; BUN, Blood urea nitrogen; CD, Cluster of differentiation; ALP, Alkaline phosphatase; ALT, Alanine aminotransferase; ROS, Reactive oxygen species; T1DM, Type 1 diabetes mellitus; AST, Aspartate aminotransferase; FBS, Fetal bovine serum; MSCs, Mesenchymal stem cells; PBS, Phosphate-buffered saline; SEM, Standard error of mean
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2021.09.067

Diabetes mellitus (DM) is one of the most serious threats in the 21th century throughout the human population that needs to be addressed cautiously. Nowadays, stem cell injection is considered among the most promising protocols for DM therapy; owing to its marked tissues and organs repair capability. Therefore, our 4 weeks study was undertaken to elucidate the probable beneficial effects of two types of adult mesenchymal stem cells (MSCs) on metabolism disturbance and some tissue function defects in diabetic rats. Animals were classified into 4 groups; the control group, the diabetic group, the diabetic group received a single dose of adipose tissue-derived MSCs and the diabetic group received a single dose of bone marrow-derived MSCs. Herein, both MSCs treated groups markedly reduced hyperglycemia resulting from diabetes induction via lowering serum glucose and rising insulin and C-peptide levels, compared to the diabetic group. Moreover, the increased lipid fractions levels were reverted back to near normal values as a consequence to MSCs injection compared to the diabetic untreated rats. Furthermore, both MSCs types were found to have hepato-renal protective effects indicated through the decreased serum levels of both liver and kidney functions markers in the treated diabetic rats. Taken together, our results highlighted the therapeutic benefits of both MSCs types in alleviating metabolic anomalies and hepato-renal diabetic complications.