Spatial Period of Laser-Induced Surface Nanoripples on PET Determines Escherichia coli Repellence

Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of...

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Published inNanomaterials (Basel, Switzerland) Vol. 11; no. 11; p. 3000
Main Authors Richter, Anja M., Buchberger, Gerda, Stifter, David, Duchoslav, Jiri, Hertwig, Andreas, Bonse, Jörn, Heitz, Johannes, Schwibbert, Karin
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 08.11.2021
MDPI
Subjects
Adhesion
Adhesion tests
Appendages
Bacteria
biofilm formation
Biofilms
Biofouling
Cell adhesion
cell appendages
Colonization
E coli
Extracellular matrix
F pili
Foils
laser processing
laser-induced periodic surface structures (LIPSS)
Lasers
Microbial contamination
Microorganisms
Oxidation
Polyethylene terephthalate
Polymers
Pulse duration
Radiation
Scanning electron microscopy
Surface chemistry
Topography
Transplants & implants
Ultraviolet lasers
Germany
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Summary:Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from ~210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for E. coli that decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient E. coli TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.
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These authors contributed equally to this article.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano11113000