Postprandial lipids accelerate and redirect nitric oxide consumption in plasma

• Published in: Nitric oxide Vol. 55-56; pp. 70 - 81
• Main Authors: Vrancken, Kurt, Schroeder, Hobe J., Longo, Lawrence D., Power, Gordon G., Blood, Arlin B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2016
• Subjects: Adult; Aged; Animals; Diet, High-Fat - adverse effects; High-fat meal; Humans; Lipid; Lipoproteins - blood; Male; Middle Aged; Nitric oxide; Nitric Oxide - blood; Nitrite; Nitrites - blood; Oxidation-Reduction; Plasma; Postprandial Period; S-nitrosothiol; S-Nitrosothiols - blood; Sheep; Triglycerides - blood; High-fat meal; Plasma; DTPA; Nitric oxide; NEM; PROLI-NO; S-nitrosothiol; Nitrite; CO; Cp; Lipid; TRL
• ISSN: 1089-8603; 1089-8611
• DOI: 10.1016/j.niox.2016.03.004

Nitric oxide (NO) and O2 are both three-to four-fold more soluble in biological lipids than in aqueous solutions. Their higher concentration within plasma lipids accelerates NO autoxidation to an extent that may be of importance to overall NO bioactivity. This study was undertaken to test the hypothesis that increased plasma lipids after a high-fat meal appreciably accelerate NO metabolism and alter the byproducts formed. We found that plasma collected from subjects after consumption of a single high-fat meal had a higher capacity for NO consumption and consumed NO more rapidly compared to fasting plasma. This increased NO consumption showed a direct correlation with plasma triglyceride concentrations (p = 0.006). The accelerated NO consumption in postprandial plasma was reversed by removal of the lipids from the plasma, was mimicked by the addition of hydrophobic micelles to aqueous buffer, and could not be explained by the presence of either free hemoglobin or ceruloplasmin. The products of NO consumption were shifted in postprandial plasma, with 55% more nitrite (n = 12, p = 0.002) but 50% less SNO (n = 12, p = 0.03) production compared to matched fasted plasma. Modeling calculations indicated that NO autoxidation was accelerated by about 48-fold in the presence of plasma lipids. We conclude that postprandial triglyceride-rich lipoproteins exert a significant influence on NO metabolism in plasma. •A major portion of plasma NO metabolism occurs in lipid micelles.•Triglyceride-rich lipoproteins following a high-fat meal increase the rate of NO consumption in isolated plasma.•Postprandial hyperlipidemia favors conversion of NO to nitrite over S-nitrosothiols in isolated plasma.