CTX-M-1-Related Extended-Spectrum Beta-Lactamases Producing Escherichia coli: so far a Sporadic Event in Western Austria

• Published in: Infection Vol. 36; no. 4; pp. 362 - 367
• Main Authors: Prelog, M, Fille, M, Prodinger, W, Grif, K, Brunner, A, Würzner, R, Zimmerhackl, L. B
• Format: Journal Article
• Language: English
• Published: Munchen Munchen : Urban and Vogel 01.08.2008; Urban and Vogel; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Anti-Infective Agents - pharmacology; Austria; Bacteremia - microbiology; Bacterial Typing Techniques; beta-Lactamases - biosynthesis; beta-Lactamases - genetics; Brief Report; Cluster Analysis; Community-Acquired Infections - microbiology; Cross Infection - microbiology; Drug Resistance, Bacterial - genetics; E coli; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Family Medicine; Female; General Practice; Humans; Infectious Diseases; Internal Medicine; Male; Medicine; Medicine & Public Health; Microbial Sensitivity Tests; Middle Aged; Plasmids - genetics; Urinary Tract Infections - microbiology; Austria; Empiric Antibiotic Therapy; Clin Microbiol Infect; Cefotaxime; Minimal Inhibitory Concentration; Antimicrob Agent
• ISSN: 0300-8126; 1439-0973; 1439-0972
• DOI: 10.1007/s15010-008-7309-7

Background: The present study was aimed to searching for CTX-M-type extended-spectrum beta-lactamases in community- and hospital-acquired Escherichia coli (E. coli) collected in western Austria and to investigate their clonal relatedness and their ability to spread. Patients and Methods: All patients with E. coli positive cultures collected from a catchment population of 186,000 between January and July 2006 were enrolled into the study. CTX-M-producing E. coli were identified by antibiotic susceptibility testing and blaCTX-M multiplex PCR. Clonal relatedness was analyzed by pulsed-field gel electrophoresis (PFGE). Results: In 2,042 E. coli isolates, 20 isolates (16 from urine, 4 from blood cultures) demonstrated CTX-M-1-related genes and no CTX-M-2- or CTX-M-9-related enzymes or CTX-M-15-producing strains were identified. We did not find clonal relatedness among CTX-M-1 producers isolated from the same referring center. E. coli were investigated for plasmid transfer ability of CTX-M-1-encoding genes. Plasmid digest patterns were not consistent with episomal spread of resistance loci. Transfection of CTX-M-encoding plasmids failed. Conclusion: Our data suggest that the emergence of CTX-M-1-producing E. coli in western Austria may be attributed to multiple independent events.