Development of glucose sensor using two-photon adsorbed photopolymerization

• Published in: Bioprocess and biosystems engineering Vol. 33; no. 1; pp. 47 - 53
• Main Authors: Kim, Jong Min, Park, Jung-Jin, Lee, Haeng-Ja, Kim, Woo-Sik, Muramatsu, Hiroshi, Chang, Sang-Mok
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Biological and medical sciences; Biosensors; Biotechnology; Blood Glucose - analysis; Chemistry; Chemistry and Materials Science; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Electroplating; Environmental Engineering/Biotechnology; Fabrication; Ferricyanides - chemistry; Food Science; Fundamental and applied biological sciences. Psychology; Glucose; Glucose Oxidase - chemistry; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Innovations; Medical technology; Microelectrodes; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Nanotechnology; Nanotubes, Carbon - chemistry; Original Paper; Photochemistry - instrumentation; Photochemistry - methods; Photons; Sensitivity and Specificity; Silver; Silver - chemistry; Silver Compounds - chemistry; Ultraviolet radiation; Carbon nanotube; Micro-flow; Potassium ferrocyanide; Glucose sensor; Two-photon adsorbed photopolymerization; Three electrode; GOD (Glucose oxidase); Glucose oxidase; Enzyme; Nanotube; Carbon; Electrodes; Detector; Photopolymerization; Oxidoreductases; Potassium
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-009-0368-z

A novel glucose sensor was constructed, and its analytical potential examined. A chip-type three-electrode system for use in a flow-type electrochemical glucose sensor was fabricated using a UV lithography technique on a glass slide. An Ag/AgCl reference electrode was made by electroplating silver onto a Pt electrode and dipping in a saturated KCl solution for 30 min. In addition, a glucose-sensing electrode was fabricated using a two-photon adsorbed photopolymerization technique with a photo-reactive resin containing a glucose oxidase enzyme, ferrocene mediator, non-ionic surfactant, and carbon nanotubes. The cyclic voltammetry of the potassium ferrocyanide in the Pt sensor system showed a stable electrode condition. The response of the modified Pt sensor confirms the feasibility of using a two-photon adsorbed photopolymerization technique for the easy fabrication of functional biosensors.