In vitro measurement of pollen tube growth inhibition

A method for estimating inhibition of pollen tube growth was developed. Pollen is placed in straight lines on an agar surface where it responds uniformly and predictably to aqueous solutions of germination-inhibiting substances located in wells at the ends of the lines. A scale of ratings, roughly c...

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Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 49; no. 6; pp. 924 - 925
Main Author Martin, F.W
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.06.1972
Subjects
Antibiotics
Calcium
Chlorides
Germination
Indoleacetic acids
Organic foods
plant biochemistry
plant physiology
Pollen
Pollen tubes
Salts
Solvents
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Summary:A method for estimating inhibition of pollen tube growth was developed. Pollen is placed in straight lines on an agar surface where it responds uniformly and predictably to aqueous solutions of germination-inhibiting substances located in wells at the ends of the lines. A scale of ratings, roughly corresponding to serial, doubled concentrations of inhibiting substances, was devised. Water-soluble organic solvents are relatively noninhibitory, salts are variable, and metabolic inhibitors have strong inhibitory effects. Pollens differ in their susceptibility to inhibition and in their response to particular substances.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.49.6.924