Relative distribution of gastrin-, CCK-8-, NPY- and CGRP-immunoreactive cells in the digestive tract of dorado ( Salminus brasiliensis )

• Published in: Tissue & cell Vol. 47; no. 2; pp. 123 - 131
• Main Authors: Pereira, R.T, Costa, L.S, Oliveira, I.R.C, Araújo, J.C, Aerts, M, Vigliano, F.A, Rosa, P.V
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.04.2015
• Subjects: Advanced Basic Science; Animals; Calcitonin - metabolism; Calcitonin Gene-Related Peptide - metabolism; Endocrine cells; Fishes - metabolism; Gastrins - metabolism; Gastrointestinal Tract - metabolism; Immunohistochemistry; Immunohistochemistry - methods; Intestine; Mixed modeling; Neuropeptide Y - metabolism; Neuropeptides; Protein Precursors - metabolism; Serotonin - metabolism; Sincalide - metabolism; Somatostatin - metabolism; Substance P - metabolism; Immunohistochemistry; PS; pyloric stomach region; FL; neuropeptide Y; cholecystokinin; Neuropeptides; first loop of medium intestine; gastrin; endocrine cells; posterior intestine; ECs; Intestine; NPY; DNES; CCK-8; SL; diffuse neuroendocrine system; calcitonin gene-related peptide; second loop of medium intestine; pyloric caeca; PC; Mixed modeling; GAS; PI; CGRP; Endocrine cells
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2015.01.009

Abstract The endocrine cells (ECs) of the gastrointestinal mucosa form the largest endocrine system in the body, not only in terms of cell numbers but also in terms of the different produced substances. Data describing the association between the relative distributions of the peptide-specific ECs in relation to feeding habits can be useful tools that enable the creation of a general expected pattern of EC distribution. We aimed to investigate the distribution of ECs immunoreactive for the peptides gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in different segments of the digestive tract of carnivorous fish dorado ( Salminus brasiliensis ) by using immunohistochemistry procedures. The distribution of endocrine cells immunoreactive for gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in digestive tract of dorado S. brasiliensis was examined by immunohistochemistry. The results describe the association between the distribution of the peptide-specific endocrine cells and feeding habits in different carnivorous fish. The largest number of endocrine cells immunoreactive for GAS, CCK-8, and CGRP were found in the pyloric stomach region and the pyloric caeca. However, NPY-immunoreactive endocrine cells were markedly restricted to the midgut. The distribution pattern of endocrine cells identified in S. brasiliensis is similar to that found in other carnivorous fishes.