Heterologous array analysis in Heterobasidion: Hybridisation of cDNA arrays with probe from mycelium of S, P or F-types

• Published in: Journal of microbiological methods Vol. 75; no. 2; pp. 219 - 224
• Main Authors: Lundén, K., Eklund, M., Finlay, R., Stenlid, J., Asiegbu, F.O.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2008; Elsevier
• Subjects: Basidiomycota - classification; Basidiomycota - genetics; Basidiomycota - metabolism; Basidiomycota - physiology; Biologi; Biological and medical sciences; Biology; Conservation; DNA Probes; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Fungal; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Heterobasidion abietinum; Heterobasidion annosum; Heterobasidion parviporum; Macroarray; Microbiology; Mycelium - classification; Mycelium - genetics; Mycelium - growth & development; Mycelium - metabolism; Mycology; NATURAL SCIENCES; NATURVETENSKAP; Oligonucleotide Array Sequence Analysis - methods; Species Specificity; Spores, Fungal - physiology; Heterobasidion parviporum; Macroarray; Heterobasidion annosum; Gene expression; Heterobasidion abietinum; Conservation; Statistical analysis; Genomics; Basidiomycota; Fungi; Polymerase chain reaction; Complementary DNA; Gene
• ISSN: 0167-7012; 1872-8359; 1872-8359
• DOI: 10.1016/j.mimet.2008.06.014

Because of the close relatedness between three species of Heterobasidion annosum (P-type), Heterobasidion parviporum (S-type) and Heterobasidion abietinum (F-type), we investigated the possible use of arrays from one species for studies of gene expression in the other. Clones containing partial cDNAs from 94 identifiable genes expressed during spore germination and differentiation in H. parviporum were printed manually in six replications on nylon membranes. The membrane was hybridized with chemifluorescent labelled cDNA from actively growing mycelia of H. parviporum, H. annosum or H. abietinum, cultivated on a non-selective substrate. Product–moment correlation coefficient varied between 0.81 and 0.49. Due to the level of correlation , in the gene expression among the intersterility groups, we concluded that the cDNA array of one can be used to study gene expression in the others.