Functional Evaluation of the Signal Peptides of Secreted Proteins
Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluate...
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| Published in | Bio-protocol Vol. 8; no. 9; p. e2839 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Bio-Protocol
05.05.2018
Bio-protocol LLC |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2331-8325 2331-8325 |
| DOI | 10.21769/BioProtoc.2839 |
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| Abstract | Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast
YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily. |
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| AbstractList | Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily. Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily. Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily. Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily. |
| Author | Chen, Tao Wang, Yufu Lin, Yang Yin, Weixiao Luo, Chaoxi |
| Author_xml | – sequence: 1 givenname: Weixiao surname: Yin fullname: Yin, Weixiao – sequence: 2 givenname: Yufu surname: Wang fullname: Wang, Yufu – sequence: 3 givenname: Tao surname: Chen fullname: Chen, Tao – sequence: 4 givenname: Yang surname: Lin fullname: Lin, Yang – sequence: 5 givenname: Chaoxi surname: Luo fullname: Luo, Chaoxi |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34286044$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/S0378-1119(97)00330-2 10.1371/journal.ppat.1005348 10.1105/tpc.109.068247 10.1038/nmeth.1701 10.1105/tpc.107.056093 10.1038/nprot.2007.15 |
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| Snippet | Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast
YTK12 strain and pSUC2 vector... Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae... Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae... |
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| Title | Functional Evaluation of the Signal Peptides of Secreted Proteins |
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