Functional Evaluation of the Signal Peptides of Secreted Proteins

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluate...

Full description

Saved in:
Bibliographic Details
Published inBio-protocol Vol. 8; no. 9; p. e2839
Main Authors Yin, Weixiao, Wang, Yufu, Chen, Tao, Lin, Yang, Luo, Chaoxi
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.05.2018
Bio-protocol LLC
Subjects
Biology
Methods
Function
Signal peptide
Secreted protein
Yeast
pSUC2 vector
Online AccessGet full text

Cover

More Information
Summary:Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2839