Using Zebrafish as a Disease Model to Study Fibrotic Disease

• Published in: International journal of molecular sciences Vol. 22; no. 12; p. 6404
• Main Authors: Wang, Xixin, Copmans, Daniëlle, de Witte, Peter A. M.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 15.06.2021; MDPI
• Subjects: Animal models; Apoptosis; Bone marrow; Cardiovascular diseases; chemical induction; Chronic illnesses; Clinical trials; Danio rerio; Disease; Drug development; Drug discovery; ECM accumulation; Extracellular matrix; Fibroblasts; Fibrosis; fibrotic disease; genetic manipulation; Immune system; In vivo methods and tests; Liver; Liver cirrhosis; Liver diseases; Pancreatitis; Pathogenesis; Review; Smooth muscle; Stem cells; Wound healing; Zebrafish
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22126404

In drug discovery, often animal models are used that mimic human diseases as closely as possible. These animal models can be used to address various scientific questions, such as testing and evaluation of new drugs, as well as understanding the pathogenesis of diseases. Currently, the most commonly used animal models in the field of fibrosis are rodents. Unfortunately, rodent models of fibrotic disease are costly and time-consuming to generate. In addition, present models are not very suitable for screening large compounds libraries. To overcome these limitations, there is a need for new in vivo models. Zebrafish has become an attractive animal model for preclinical studies. An expanding number of zebrafish models of human disease have been documented, for both acute and chronic diseases. A deeper understanding of the occurrence of fibrosis in zebrafish will contribute to the development of new and potentially improved animal models for drug discovery. These zebrafish models of fibrotic disease include, among others, cardiovascular disease models, liver disease models (categorized into Alcoholic Liver Diseases (ALD) and Non-Alcoholic Liver Disease (NALD)), and chronic pancreatitis models. In this review, we give a comprehensive overview of the usage of zebrafish models in fibrotic disease studies, highlighting their potential for high-throughput drug discovery and current technical challenges.