Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture

• Published in: International journal of molecular sciences Vol. 23; no. 13; p. 7277
• Main Authors: Nogueira, Lucas Fabrício Bahia, Eufrásio Cruz, Marcos Antônio, Aguilar, Guilherme José, Tapia-Blácido, Delia Rita, da Silva Ferreira, Márcia Eliana, Maniglia, Bianca Chieregato, Bottini, Massimo, Ciancaglini, Pietro, Ramos, Ana Paula
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.07.2022; MDPI
• Subjects: Antiinfectives and antibacterials; Antimicrobial agents; Biocompatibility; Biodegradation; Biological activity; Biomedical materials; Biomimetics; biomineralization; Biopolymers; Body fluids; Bone composition; Bone growth; Calcium phosphates; Carrageenan; Chemical precipitation; Chemicals; Chitosan; Chloride; Collagen; Confined spaces; Fibrillogenesis; Glycerol; hybrid membranes; Hydrogels; Hydroxyapatite; Incorporation; Interstices; Maxillofacial surgery; Mechanical properties; Membranes; Methodology; Microorganisms; Mineralization; Nanoparticles; Osteoblasts; Osteogenesis; Polysaccharides; Silver; Sodium; Tissue engineering; Brazil; United States > US
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23137277

Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition. The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2). The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr). The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties. The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1. HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive. Hybrid membranes containing Cht were not toxic to the osteoblasts. Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms. Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.