Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis

• Published in: Biosensors (Basel) Vol. 11; no. 8; p. 264
• Main Authors: Wang, Chunxue, Yi, Pengfei, Li, Jiang, Dong, Haibing, Chen, Changming, Zhang, Daming, Shen, Haiqing, Fu, Bendong
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 06.08.2021; MDPI
• Subjects: Apoptosis; Biosensors; Cell cycle; Chinese medicine; Communication; Design; effective drug concentrations; Endothelial cells; Fluorination; Genes; Ginkgolides; Herbal medicine; Microvasculature; Optical waveguides; Photosensitivity; polymer optical waveguide; Polymers; Polymethyl methacrylate; Polymethylmethacrylate; Refractivity; Sensors; Software; waveguide grating-based biosensor; China
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios11080264

In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10−5 RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients.