Microbial N-demethylation: biotransformation and recovery of a drug metabolite

A total of 39 microbes were screened for the ability to selectively N-demethylate (3R,5S,E)-7-(4-(4-fluorophenyl)-6-isopropyl-2-(methyl(1-methyl-1H-1,2,4-triazol-5-yl)aminopyrimidin-5-yl)-3,5-dihydroxy-hept-6-enoic acid (I), a potential drug for lowering blood cholesterol levels. Two Streptomyces sp...

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Bibliographic Details
Published inBiotechnology and applied biochemistry Vol. 53; no. Pt 2; p. 133
Main Authors Davis, Brian L, Liu, Mark S, Hanson, Ronald L, Parker, William L, Patel, Ramesh N
Format Journal Article
LanguageEnglish
Published United States 01.06.2009
Subjects
Bacteria - metabolism
Biotransformation
Fungi - metabolism
Methylation
Pyrimidines - metabolism
Streptomyces - metabolism
Triazoles - metabolism
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Summary:A total of 39 microbes were screened for the ability to selectively N-demethylate (3R,5S,E)-7-(4-(4-fluorophenyl)-6-isopropyl-2-(methyl(1-methyl-1H-1,2,4-triazol-5-yl)aminopyrimidin-5-yl)-3,5-dihydroxy-hept-6-enoic acid (I), a potential drug for lowering blood cholesterol levels. Two Streptomyces species were found to carry out the desired N-demethylation. Bioconversion by Streptomyces griseus A.T.C.C. 13273 and product recovery were scaled up to the multi-gram level.
ISSN:1470-8744
DOI:10.1042/BA20080079