Intestinal colonization of a human subject by vancomycin-resistant Enterococcus faecium

• Published in: Clinical microbiology and infection Vol. 5; no. 2; pp. 97 - 100
• Main Author: Berchieri Jr, Angelo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.02.1999; Blackwell Publishing Ltd; Blackwell; Elsevier Limited
• Subjects: Antibacterial agents; Antibiotic resistance; Antibiotics. Antiinfectious agents. Antiparasitic agents; avoparcin; Biological and medical sciences; Enterococcus faecium; intestinal colonization; Medical sciences; Pharmacology. Drug treatments; vancomycin resistance; VRE; VRE; Enterococcus faecium; intestinal colonization; vancomycin resistance; avoparcin; Human; Peptides; Gut; Vancomycin; Experimental study; Infection; Case study; Streptococcaceae; Antibiotic; Chemotherapy; Treatment; Glycopeptide; Bacteriosis; Digestive diseases; Bacteria; Intestinal disease; Micrococcales; Antibacterial agent; Negative therapeutic reaction; Colonization
• ISSN: 1198-743X; 1469-0691; 1469-0691
• DOI: 10.1111/j.1469-0691.1999.tb00110.x

To study the ability of two strains of vancomycin-resistant Enterococcus faecium to colonize the human intestine. A single human subject ingested separately two strains of vancomycin-resistant E. faecium isolated from a pig and a chicken. The feces were cultured on selective medium. Prior to ingestion no vancomycin-resistant cocci were present in the feces. Ingestion of 104–105 CFU resulted in either no colonization or isolation only after enrichment. Ingestion of 107 CFU of one strain resulted in colonization for a period of nearly 3 weeks, with fecal counts at times in excess of 106 CFU/g. Ingestion of similar numbers of the other strain and reingestion of the first strain resulted in excretion in the feces for much shorter periods. When the fecal count of the ingested strains was greater than 104–105 CFU/g, the strains were isolated from swabs taken from perianal skin. Vancomycin-resistant E. faecium strains from pigs and poultry are able to colonize the human gut and the perianal skin.