Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells

• Published in: Cell biology international Vol. 18; no. 11; pp. 1059 - 1065
• Main Authors: Kaeffer, B., Uriel, I.Garcia, Bottreau, E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.11.1994; Blackwell Publishing Ltd; Wiley
• Subjects: Animals; Antigens, Polyomavirus Transforming - biosynthesis; Cell Line, Transformed; Cell Survival; Cold Temperature; Cricetinae; Cricetulus - anatomy & histology; Cryopreservation; Cryoprotective Agents - pharmacology; Culture Techniques - methods; Epithelial Cells; Female; Fibroblasts - cytology; Hamster; Hypothermia; Ileum - cytology; Intestinal Mucosa - cytology; Keratins - biosynthesis; Life Sciences; Minipig; Minipig, Hamster, Hypothermia, Cryopreservation; Neutral Red; Ovary - cytology; Simian virus 40 - genetics; Species Specificity; Swine; Swine, Miniature - anatomy & histology; Minipig, Hamster, Hypothermia, Cryopreservation
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1006/cbir.1994.1029

Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25°C and 3 hours recovery at 37°C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-I fibroblasts. Epithelioid cells survived 3 days at 25°C with unaltered expression of cytokeratin-18 whereas colonies of fibroblasts did not survive more than a day at 25°C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.