Effect of Cinnamoyl and Flavonol Glucosides Derived from Cherry Blossom Flowers on the Production of Advanced Glycation End Products (AGEs) and AGE-induced Fibroblast Apoptosis

• Published in: Phytotherapy research Vol. 25; no. 9; pp. 1328 - 1335
• Main Authors: Shimoda, Hiroshi, Nakamura, Seiko, Morioka, Makoto, Tanaka, Junji, Matsuda, Hisashi, Yoshikawa, Masayuki
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2011; Wiley
• Subjects: advanced glycation end product; Apoptosis - drug effects; Biological and medical sciences; Caffeic Acids - pharmacology; caffeoyl glucose; Cell Line; cherry blossom; Collagen - metabolism; collagen lattice; fibroblast; Fibroblasts - drug effects; Flavonols - pharmacology; Flowers - chemistry; General pharmacology; Glucosides - pharmacology; Glycation End Products, Advanced - metabolism; Humans; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - pharmacology; Prunus - chemistry; Quercetin - analogs & derivatives; Quercetin - pharmacology; quercetin glucoside; Glucose; Flavonoid; Medicinal plant; caffeoyl glucose; Polyphenol; Glycoside; Age; advanced glycation end product; Flower; Aldose; Pharmacognosy; Glycoprotein; Glucoside; Protein; collagen lattice; Cell death; Collagen; Cinnamic acid derivatives; Plant origin; Quercetin; Phenols; cherry blossom; Fibroblast; Apoptosis; quercetin glucoside; fibroblast
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.3423

Cherry blossom flowers are familiar to the Japanese, and some species of the flowers soaked in salty vinegar are used as processed foods. The constituents of aqueous ethanol extract from cherry blossom (Prunus lannesiana) flowers (CBE) were examined and cinnamoyl and flavonol glucosides were isolated. To elucidate the pharmacological functions of CBE and its constituents, their effects on the production of advanced glycation end products (AGEs) and on AGE‐induced fibroblast damage were examined. CBE and 1‐O‐(E)‐caffeoyl‐β‐d‐glucopyranoside (CaG), a principal compound in CBE, significantly suppressed the production of AGEs derived from glucose and albumin at 100 μg/mL. Among the flavonol glucosides, quercetin 3‐O‐β‐d‐glucopyranoside (QG) exhibited potent suppressive activity (IC50: 30 μg/mL). CBE and CaG suppressed glyoxal‐induced AGE production in fibroblasts at 10 μg/mL, but QG did not. In addition, CBE and CaG recovered collagen lattice formation consisting of collagen and glycated fibroblasts at 10 μg/mL. Moreover, CBE and its constituents, except kaempferol 3‐O‐(6″‐malony)‐β‐d‐glucopyranoside, significantly suppressed fibroblast apoptosis induced by carboxymethyl lysine‐collagen at 10 μg/mL. These results show that cinnamoyl and flavonol glucosides of cherry blossom flowers suppress AGE production and AGE‐induced fibroblast apoptosis. Cherry blossom flowers may be effective against skin AGE production and fibroblast damage by AGEs. Copyright © 2011 John Wiley & Sons, Ltd.