Association of cyclin D1 gene polymorphisms with risk of esophageal squamous cell carcinoma in Kashmir Valley-A high risk area

• Published in: Molecular carcinogenesis Vol. 50; no. 7; pp. 487 - 498
• Main Authors: Hussain, Showket, M., Yuvaraj, Thakur, Nisha, Salam, Irfana, Singh, Neha, Mir, Mohammad Muzaffar, Bhat, Mohammad Akbar, Siddiqi, Mushtaq A., Das, Bhudev C., Bharadwaj, Mausumi
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.2011; Wiley Subscription Services, Inc
• Subjects: Adult; Carcinogenesis; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - genetics; CCND1; cyclin D1; Cyclin D1 - genetics; Electrophoresis, Polyacrylamide Gel; esophageal cancer; Esophageal Neoplasms - epidemiology; Esophageal Neoplasms - genetics; Esophagus; Female; Gene polymorphism; Genetic Predisposition to Disease; Genotype; Genotyping; Humans; India - epidemiology; Kashmir; Male; Middle Aged; Natural sciences; Naturvetenskap; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Restriction fragment length polymorphism; Risk Factors; Single-nucleotide polymorphism; Smoking; squamous cell carcinoma; Tea; India; Kashmir; gene polymorphism
• ISSN: 0899-1987; 1098-2744; 1098-2744
• DOI: 10.1002/mc.20732

Investigation of potential association of SNPs (G870A, rs9344; G1722C, rs678653) of cyclin D1 gene (CCND1) with susceptibility to esophageal squamous cell carcinoma (ESCC) in Kashmir valley (India). The study included 302 subjects comprising 151 ESCC cases and 151 controls. PCR‐RFLP and direct sequencing were employed for genotyping. The G870A polymorphism, the individuals carrying GA + AA genotype was having 2.80‐fold increased risk for development of ESCC (OR 2.8, 95% CI = 1.77–4.4; P = 0.0001) compared to GG genotype. Further a significantly higher risk was observed in individuals who consume >3 cups per day of salted tea (OR = 5.1; 95% CI = 1.6–16.7; P = 0.0016) and had smoking habits (OR = 6.3; 95% CI = 2.9–13.9; P = 0.0005). We also demonstrate for the first time in CCND1 1722 locus, the CC genotype was strongly associated with increased risk of developing ESCC (OR = 2.58; 95% CI = 1.61–4.15; P = 0.0001). In addition, the frequency of polymorphic C allele was also found to be higher in cases (OR = 1.92; 95% CI = 1.37–2.69; P = 0.0002). There appears to be an influence of CCND1 G870A/G1772C genotypes on genetic susceptibility to ESCC. Mol. Carcinog. ©2011 Wiley‐Liss, Inc.