Actions of N-acetylcysteine, daptomycin, vancomycin, and linezolid on methicillin-resistant Staphylococcus aureus biofilms in the ventriculoperitoneal shunt infections: an experimental study

• Published in: Chinese neurosurgical journal Vol. 8; no. 1; pp. 1 - 15
• Main Authors: Kuruoglu, Tuba, Altun, Gamze, Kuruoglu, Enis, Turan, Derya Bayırlı, Önger, Mehmet Emin
• Format: Journal Article
• Language: English
• Published: London BioMed Central 05.07.2022; BMC
• Subjects: Antibiotic-impregnated catheters; Antibiotics; Antimicrobial agents; Biofilm; Biofilms; Catheters; Gram-positive bacteria; Hydrocephalus; Methicillin-resistant Staphylococcus aureus; Pathogens; Scanning electron microscope; Staphylococcus infections; Statistical analysis; Surgery; Istanbul Turkey; Germany; Turkey
• ISSN: 2057-4967; 2095-9370; 2057-4967
• DOI: 10.1186/s41016-022-00284-2

Abstract Background Shunt systems are used to provide cerebrospinal fluid drainage in the treatment of hydrocephalus. Recently, antibiotic-impregnated shunt systems are used to prevent colonization in the ventriculoperitoneal catheters. Methicillin-resistant Staphylococcus aureus (MRSA) is the most common causative microorganism of shunt infections. The aim of the study is to investigate effects of several substances on MRSA biofilms in the ventriculoperitoneal catheters. Methods The present study consists of mainly eight groups (each has two subgroups as antibiotic-impregnated and nonantibiotic-impregnated catheters). In addition, each group contains six molds using MRSA strains. In this study, daptomycin (DAPT) (2 mg/ml), vancomycin (VAN) (10 mg/ml), linezolid (LIN) (2 mg/ml), N-acetylcysteine (NAC) (6 mg/ml), and various combinations of these substances were used to evaluate the treatment against MRSA using scanning electron microscope (SEM) images and microbiological enumeration. Results The colony count in the antibiotic-impregnated samples significantly decreased compared to nonantibiotic-impregnated samples in the MRSA, MRSA + DAPT, and MRSA + LIN groups ( p  < 0.01), respectively. Conversely, the colony count in antibiotic-impregnated samples significantly increased compared to nonantibiotic-impregnated samples in NAC + DAPT and NAC + VAN groups ( p  < 0.01), respectively. Conclusions The results showed that the use of antibiotic-impregnated catheters has a significant impact on the prevention of infection whereas the combination of NAC and DAPT showed better antibiofilm and antibacterial effects than other combinations on the prevention and treatment of nonantibiotic-impregnated catheter infections.