Modulation of rat neutrophil functions by administration of granulocyte colony-stimulating factor

• Published in: Journal of leukocyte biology Vol. 57; no. 2; pp. 250 - 256
• Main Authors: Murata, Kotaro, Watanabe, Tadashi, Yamashita, Takao, Gon, Songi, Sendo, Fujiro
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.02.1995
• Subjects: adherence; Amino Acid Sequence; Animals; Ascitic Fluid - cytology; Buffers; Cell Adhesion - drug effects; Cell Division - drug effects; fMLP; Granulocyte Colony-Stimulating Factor - pharmacology; Male; Molecular Sequence Data; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - drug effects; Neutrophils - metabolism; Neutrophils - physiology; peripheral blood neutrophil; peritoneal exudate neutrophil; phorbol myristate acetate; Phosphates; Rats; Rats, Wistar; superoxide production; Superoxides - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Tumor Cells, Cultured - drug effects; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.57.2.250

Neutrophil function was examined in rats treated with recombinant human granulocyte colony‐stimulating factor (G‐CSF) using peripheral blood neutrophils (PBNs) and peritoneal exudate neutrophils (PENs) as sources of cells examined in vitro. Adherence to plastic plates containing fetal calf serum of nonstimulated or N‐formylmethionyl‐leucyl‐phenylalanine (fMLP)‐ or tumor necrosis factor‐α‐stimulated PBNs obtained from G‐CSF‐injected rats was lower than that of control rats. In contrast, this adherence was higher in G‐CSF‐treated rats than in the control group when PENs were used. Neutrophil adherence of G‐CSF‐injected and noninjected groups was identical when phorbol myristate acetate was used to stimulate neutrophils. Superoxide production of PBNs stimulated with fMLP in vitro was lower in G‐CSF‐treated rats than in control rats but higher than in the controls when PENs were used. Furthermore, in vitro tumor cell growth inhibition by PBNs was lower in G‐CSF‐treated rats than in control rats, but when PENs were used inhibition was higher than in the controls. J. Leukoc. Biol. 57: 250–256; 1995.