Effects of soy isoflavone consumption on bone structure and milk mineral concentration in a rat model of lactation-associated bone loss

• Published in: European journal of nutrition Vol. 48; no. 2; pp. 84 - 91
• Main Authors: Peterson, Catherine A. PhD, RD, Schnell, Jennifer D. MS, Kubas, Karen L. MS, Rottinghaus, George E. PhD
• Format: Journal Article
• Language: English
• Published: Darmstadt Darmstadt : Steinkopff-Verlag 01.03.2009; Steinkopff-Verlag; Springer; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Bone and Bones - pathology; Calcium - analysis; Chemistry; Chemistry and Materials Science; Diet; Disease Models, Animal; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Genistein - analysis; Genistein - blood; Glycine max - chemistry; Isoflavones - administration & dosage; Lactation; Milk - chemistry; Minerals - analysis; Nutrition; Original Contribution; Osteoporosis - etiology; Osteoporosis - metabolism; Osteoporosis - pathology; Phosphorus - analysis; Random Allocation; Rats; Rats, Wistar; Vertebrates: anatomy and physiology, studies on body, several organs or systems; rat model; isoflavones; genistein; lactation; bone loss; Animal model; Lactation; Rat; Genistein; Diseases of the osteoarticular system; Minerals; Soybean; Flavonoid; Inorganic element; Phytoestrogen; Osteoarticular system; Polyphenol; Proanthocyanidin; Osteopenia; Milk; Isoflavone; Nutrition; Rodentia; Concentration; Antioxidant; Feeding; Micronutrient; Grain legume; Vertebrata; Mammalia; Food intake; Animal; Bone
• ISSN: 1436-6207; 1436-6215
• DOI: 10.1007/s00394-008-0765-0

Background Like menopause, during complete lactation, circulating estrogen concentrations are markedly reduced, resulting in amplified bone resorption. Aim of study To investigate the effects of soy isoflavones, common dietary components used to mitigate the bone loss of menopause, on the bone loss associated with lactation. Methods Lactating rats were randomized to one of four diets supplemented with different levels of soy isoflavones (0, 2, 4, 8 mg aglycone isoflavone/g protein). Milk was collected from all dams between days 12 and 15 of lactation and was analyzed for calcium, phosphorus and genistein concentrations. Serum and bones from half of the animals from each diet group were taken at weaning and from the remaining half at 4 weeks post-weaning. Bones underwent histomorphometric analysis and serum was used for genistein determinations. Results Serum genistein and milk concentrations reflected dietary isoflavone dose. Isoflavone intake had no effect on any of the bone changes associated with lactation or recovery. Milk calcium and mineral concentrations were unaffected by dietary isoflavones. Conclusions Consumption of soy isoflavones, in levels that can be readily attained through soy foods, have neither protective effects on bone nor deleterious effects on milk quality or quantity during lactation.