Effect of N oxyanions on anaerobic induction of nitrate reductase in subcellular fractions of Bradyrhizobium sp. (Lupinus)

• Published in: Antonie van Leeuwenhoek Vol. 95; no. 2; pp. 159 - 164
• Main Authors: Polcyn, Władysław, Luciński, Robert
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.02.2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Action of physical and chemical agents on bacteria; Activity patterns; Anaerobiosis; Anions - metabolism; Antibodies; Bacteria; Bacterial Proteins - analysis; Bacteriology; Biological and medical sciences; Biomedical and Life Sciences; Bradyrhizobium; Bradyrhizobium - chemistry; Bradyrhizobium - enzymology; Enzymatic activity; Enzyme Induction; Enzymes; Fundamental and applied biological sciences. Psychology; Life Sciences; Lupinus; Lupinus - microbiology; Medical Microbiology; Membranes; Metabolism. Enzymes; Microbiology; Nitrate; Nitrate reductase; Nitrate Reductase - metabolism; Nitrates; Nitrates - metabolism; Nitrite; Nitrites - metabolism; Original Paper; periplasm; Plant Sciences; Protein biosynthesis; Protein synthesis; Proteins; Soil Science & Conservation; Spheroplasts; Studies; Subcellular Fractions - metabolism; Time Factors; Western blotting; sp.; Membrane-bound nitrate reductase; Nitrate and nitrite induction; ); Nitrate respiration; Anaerobiosis; Enzyme; Induction; Cell fraction; Nitrate reductase; Bacteria; Oxidoreductases; Rhizobiaceae; Bradyrhizobium sp. (Lupinus); Protein content; Bradyrhizobium
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-008-9299-y

Anaerobic induction of nitrate reductase in subcellular fractions of Bradyrhizobium sp. strain USDA 3045 showed fivefold increase of the enzyme activity in spheroplasts, considered as the source of intact-membrane-bound nitrate reductase, within a 3 h time frame after nitrate addition. Such a dynamics was confirmed at the protein level, with antibodies specific to membrane-bound nitrate reductase. Nitrate reductase activity in the periplasm was one order of magnitude lower and significant only at initial 3 h of induction, within a narrow range of nitrate added. Nitrite induced the membrane-bound nitrate reductase at least 70% as effectively as nitrate, as judged from its activity pattern and Western blot analysis. The limited ability of Bradyrhizobium sp. to dissimilate >=5 mM nitrate is not due to direct inhibition of respiratory nitrate reductase by accumulated nitrite. Moreover, a synergistic induction of membrane-bound nitrate reductase by nitrate and nitrite was indicated due to a twofold higher protein synthesis after simultaneous addition of these N oxyanions than when they were given separately.