Cardiolipin Remodeling by ALCAT1 Links Oxidative Stress and Mitochondrial Dysfunction to Obesity

• Published in: Cell metabolism Vol. 12; no. 2; pp. 154 - 165
• Main Authors: Li, Jia, Romestaing, Caroline, Han, Xianlin, Li, Yuan, Hao, Xinbao, Wu, Yinyuan, Sun, Chao, Liu, Xiaolei, Jefferson, Leonard S., Xiong, Jingwei, LaNoue, Kathryn F., Chang, Zhijie, Lynch, Christopher J., Wang, Huayan, Shi, Yuguang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.08.2010
• Subjects: Acyltransferases - deficiency; Acyltransferases - genetics; Acyltransferases - metabolism; Animals; Cardiolipins - metabolism; Cell Line; HUMDISEASE; Insulin Resistance; Mice; Mitochondria - metabolism; Mitochondria - physiology; Obesity - etiology; Obesity - metabolism; Oxidative Stress; Phosphorylation; Reactive Oxygen Species - metabolism; SIGNALING; Up-Regulation; HUMDISEASE; SIGNALING
• ISSN: 1550-4131; 1932-7420
• DOI: 10.1016/j.cmet.2010.07.003

Oxidative stress causes mitochondrial dysfunction and metabolic complications through unknown mechanisms. Cardiolipin (CL) is a key mitochondrial phospholipid required for oxidative phosphorylation. Oxidative damage to CL from pathological remodeling is implicated in the etiology of mitochondrial dysfunction commonly associated with diabetes, obesity, and other metabolic diseases. Here, we show that ALCAT1, a lyso-CL acyltransferase upregulated by oxidative stress and diet-induced obesity (DIO), catalyzes the synthesis of CL species that are highly sensitive to oxidative damage, leading to mitochondrial dysfunction, ROS production, and insulin resistance. These metabolic disorders were reminiscent of those observed in type 2 diabetes and were reversed by rosiglitazone treatment. Consequently, ALCAT1 deficiency prevented the onset of DIO and significantly improved mitochondrial complex I activity, lipid oxidation, and insulin signaling in ALCAT1−/− mice. Collectively, these findings identify a key role of ALCAT1 in regulating CL remodeling, mitochondrial dysfunction, and susceptibility to DIO. ► ALCAT1 expression is upregulated by oxidative stress and the onset of diet-induced obesity ► ALCAT1 catalyzes the synthesis of cardiolipin species commonly found in metabolic diseases ► ALCAT1 causes oxidative stress and mitochondrial dysfunction when overexpressed in C2C12 cells ► ALCAT1−/− mice are resistant to DIO and the related mitochondrial defects and oxidative stress