Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

• Published in: Journal of visualized experiments no. 88
• Main Authors: Francis, Michael, Waldrup, Josh, Qian, Xun, Taylor, Mark S.
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 16.06.2014
• Subjects: Algorithms; Animals; automation; Basic Protocol; calcium; Calcium - analysis; Calcium - chemistry; Calcium - metabolism; calcium signaling; Calcium Signaling - physiology; computer software; dyes; Fluorescent Dyes - analysis; Fluorescent Dyes - chemistry; image analysis; microscopy; Microscopy, Fluorescence - methods; quantitative analysis; Signal Processing, Computer-Assisted; Software; statistical analysis; Swine

Intracellular Ca(2+) signals are commonly studied with fluorescent Ca(2+) indicator dyes and microscopy techniques. However, quantitative analysis of Ca(2+) imaging data is time consuming and subject to bias. Automated signal analysis algorithms based on region of interest (ROI) detection have been...