In vitro antileukaemic activity of extracts from berry plant leaves against sensitive and multidrug resistant HL60 cells

• Published in: Cancer letters Vol. 236; no. 2; pp. 282 - 291
• Main Authors: Skupień, Katarzyna, Oszmiański, Jan, Kostrzewa-Nowak, Dorota, Tarasiuk, Jolanta
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 18.05.2006; Elsevier Limited
• Subjects: Acids; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Antioxidants; Apoptosis; ATP-Binding Cassette, Sub-Family B, Member 1 - biosynthesis; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics; Berries; Berry pants; Blueberry Plants; Cancer; Cancer therapies; Cell culture; Cell growth; Cell Proliferation - drug effects; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Ellagic Acid - analysis; Fragaria; Free radicals; Fruits; Gallic Acid - analysis; HL-60 Cells; Human promyelocytic HL60 cells; Humans; In vitro antileukaemic activity; Inhibitory Concentration 50; Leaf extracts; Multidrug resistance MDR; Multidrug Resistance-Associated Proteins - biosynthesis; Multidrug Resistance-Associated Proteins - genetics; Phenolic compounds; Phenols - analysis; Phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Polyphenols; Quercetin - analysis; Studies; Tea; Vegetables; Leaf extracts; Multidrug resistance MDR; Phenolic compounds; Berry pants; In vitro antileukaemic activity; Human promyelocytic HL60 cells
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2005.05.018

The aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves ( Fragaria x ananassa Duch. cv Elsanta, raspberry Rubus ideus L. cv Polana and blueberry Vaccinium corymbosum L. cv Bluecrop) against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the blueberry extract was the most efficient against sensitive HL60 cell line (about 2-fold more active than strawberry and raspberry extracts) but presented much lower activity towards resistant cells. In contrast, strawberry and raspberry extracts exhibited the high cytotoxic activity against sensitive leukaemia HL60 cell line as well as its MDR sublines. The values of resistance factor (RF) found for these extracts were very low lying in the range 0.32÷2.0.