Temporal and spatial appearance of the membrane cytoskeleton and perineuronal nets in the rat neocortex

• Published in: Neuroscience letters Vol. 209; no. 3; pp. 173 - 176
• Main Authors: Wintergerst, E.S., Weisenhorn, D.M.Vogt, Rathjen, F.G., Riederer, B.M., Lambert, S., Celio, M.R.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 17.05.1996
• Subjects: Animals; Cerebral Cortex - cytology; Cerebral Cortex - growth & development; Cytoskeletal Proteins - biosynthesis; Cytoskeleton; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; Extracellular matrix; Extracellular Matrix Proteins - biosynthesis; Female; Immunohistochemistry; Interneurons - physiology; Nerve Net - anatomy & histology; Nerve Net - growth & development; Perineuronal nets; Rats; Rats, Inbred Strains; Tenascin - biosynthesis; Time Factors; Extracellular matrix; Cytoskeleton; Perineuronal nets
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(96)12643-4

Parvalbumin-immunoreactive interneurons are surrounded by perineuronal nets, containing molecules of the extracellular matrix (e.g. tenascin-R). Furthermore, they seem to have a special cytoskeleton composed of, among others, ankyrin R and β Rspectrin. In the present developmental study we showed that the intracellular markers parvalbumin, ankyrin R and β Rspectrin Villosa agglutinin, an extracellular marker for perineuronal nets, appeared in the second postnatal week. In the third postnatal week, ankyrin R and β Rspectrin were present in the parvalbumin-positive interneurons. Tenascin-R appeared in a similar topographic distribution as the intracellular markers. The adult pattern was established upon the end of the fourth postnatal week. Our results indicate that cytoskeletal maturity maybe a prerequisite for the organization of perineuronal nets of extracellular matrix.