Preparation of monoclonal antibodies against Marek's disease virus serotype 1 glycoprotein D expressed by a recombinant baculovirus

• Published in: Virus research Vol. 38; no. 2; pp. 219 - 230
• Main Authors: Ono, Mitsuru, Jang, Hyung-Kwan, Maeda, Ken, Kawaguchi, Yasushi, Tohya, Yukinobu, Niikura, Masahiro, Mikami, Takeshi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.1995
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibodies, Viral - biosynthesis; Antibodies, Viral - immunology; Antigens, Viral - genetics; Antigens, Viral - immunology; baculovirus; Baculovirus recombinant; Base Sequence; Cell Line; Chick Embryo; DNA, Viral; Genetic Vectors; Glycoprotein D; Herpesvirus 2, Gallid - immunology; Marek's disease virus serotype 1; Molecular Sequence Data; Monoclonal antibody; Nucleopolyhedrovirus - genetics; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Serotyping; Spodoptera; Spodoptera frugiperda; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Glycoprotein D; Marek's disease virus serotype 1; Monoclonal antibody; Baculovirus recombinant
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/0168-1702(95)00063-V

A recombinant baculovirus, the genome of which contains DNA encoding Marek's disease virus serotype 1 (MDV1) homolog of glycoprotein D (gD) of herpes simplex virus under the polyhedrin promoter was constructed and designated rAcMDV1gD. Five monoclonal antibodies (MAbs) which recognize the MDV1 homolog of gD (MDV1 gD) in Spodoptera frugiperda cells infected with rAcMDV1gD were prepared. The MAbs reacted with proteins ranging from 52 to 49 kDa in rAcMDV1gD-infected cell lysates by immunoblot analysis. These molecular weights were coincident with molecular weights predicted from the open reading frame of MDV1 gD. By ELISA additivity test, the 5 MAbs were divided into 3 groups which seemed to recognize 3 different epitopes. In addition, all of the 5 MAbs were reactive with chick embryo fibroblasts (CEFs) expressing MDV1 gD. The MAbs are considered to be useful to study the role of MDV1 gD in MDV1 infection.