Responses of glutamate cysteine ligase and glutathione to oxidants in deer mice ( Peromyscus maniculatus)

• Published in: Ecotoxicology and environmental safety Vol. 72; no. 5; pp. 1572 - 1578
• Main Authors: Wu, Hongmei, McBride, Tobias J., Isanhart, John P., Cox, Stephen B., Hooper, Michael J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2009
• Subjects: Aging; Animals; Brain - drug effects; Brain - enzymology; Carbon tetrachloride; Carbon Tetrachloride - administration & dosage; Carbon Tetrachloride - toxicity; Copper - toxicity; Deer mouse; Female; Glutamate cysteine ligase; Glutamate-Cysteine Ligase - blood; Glutamate-Cysteine Ligase - metabolism; Glutathione; Glutathione - blood; Glutathione - metabolism; Injections, Intraperitoneal; Kidney - drug effects; Kidney - enzymology; Lead - toxicity; Liver - drug effects; Liver - enzymology; Male; Metal; Metals, Heavy - toxicity; Myocardium - enzymology; Oxidants - administration & dosage; Oxidants - toxicity; Oxidative Stress - drug effects; Peromyscus; Peromyscus maniculatus; Risk Assessment; Soil Pollutants - toxicity; Zinc - toxicity; Metal; Glutamate cysteine ligase; Deer mouse; Carbon tetrachloride; Glutathione
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2009.02.008

Sensitivities of a wildlife species, deer mice, to oxidants were evaluated. A single dose (1589 mg/kg body weight by intraperitoneal injection) of carbon tetrachloride, a typical hepatotoxicant, caused changes in GCL activity and GSH content in multiple organs of deer mice. Hepatic GCL activity and GSH content were depleted substantially ( P<0.01), renal GCL activity increased ( P<0.05). Blood, brain and heart GCL activities increased ( P<0.05), whereas GSH contents decreased significantly. Deer mice were exposed to Pb, or Pb together with Cu and Zn via drinking water for 4 weeks. GCL activities were not significantly affected by treatments. GSH contents were increased significantly by Pb alone, Pb with medium and high concentrations of Cu and Zn. Effects of multi-metal-contaminated soil were investigated via lactational, juvenile and lifelong exposure to feed supplemented with soils. Metal-contaminated soils did not lead to significant effects in pups via lactation, 50-day exposure altered GSH content marginally, while 100-day exposure resulted in marked GCL activity depletion. After 100-day exposure, GCL activities of the medium soil-, high soil- and Pb-treated deer mice were only 53%, 40% and 46% of the control, respectively ( P<0.0001).