Exploring the effects of treatments with carbohydrases to obtain a high-cellulose content pulp from a non-wood alkaline pulp
•A high reduction (82%) in hemicelluloses content was achieved with treatments.•Direct admixture of xylanase provided better results, with 25% xylan reduction.•Cx appeared as a new very promising catalyst with both used enzymatic activities.•Enzymes catalytic mechanism was assessed via HPLC analysis...
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Published in | Carbohydrate polymers Vol. 133; pp. 302 - 312 |
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Main Authors | , , , |
Format | Journal Article Publication |
Language | English |
Published |
England
Elsevier Ltd
20.11.2015
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Subjects | |
Online Access | Get full text |
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Summary: | •A high reduction (82%) in hemicelluloses content was achieved with treatments.•Direct admixture of xylanase provided better results, with 25% xylan reduction.•Cx appeared as a new very promising catalyst with both used enzymatic activities.•Enzymes catalytic mechanism was assessed via HPLC analysis of effluents.•Enzymatic treatments produced up to a 110% increase in pulp reactivity.
In this work, treatments with a xylanase (X) and carbohydrases mixture (Cx) were applied on a TCF bleached sisal pulp in order to obtain high-cellulose content fibers applicable on a wide range of uses. A limit of ≈12% w/w final content in hemicelluloses was found regardless of the enzymatic treatment assessed. An extraction with 4% and 9% w/v NaOH was performed for further hemicelluloses removal. We found that NaOH dose could be strongly reduced if combined with Cx or Cx+X treatments. Also, if necessary, a stronger reduction could be obtained with 9% w/v NaOH, which was found to be boosted in a 14% if performed after a treatment with Cx. An end-product with a low content in xylans (≈2.9% w/w) and in HexA (5.8μmol/odp) was obtained. Pulp Fock solubility was also increased (≈30%) by enzymatic treatments. HPLC analysis of effluents provided useful information of enzymatic catalytic mechanisms. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0144-8617 1879-1344 |
DOI: | 10.1016/j.carbpol.2015.07.016 |